Induction of apoptosis in MCF-7 cells by β-1,3-xylooligosaccharides prepared from Caulerpa lentillifera

Biosci Biotechnol Biochem. 2012;76(5):1032-4. doi: 10.1271/bbb.120016. Epub 2012 May 7.

Abstract

β-1,3-Xylan was prepared from the green alga, Caulerpa lentillifera, and hydrolyzed to oligosaccharides by a mild acid treatment. The average degree of polymerization was about 5. The oligosaccharides reduced the number of viable human breast cancer MCF-7 cells in a dose-dependent manner, and induced chromatin condensation and degradation of poly ADP-ribose polymerase, indicating that they induced apoptosis in MCF-7 cells.

MeSH terms

  • Antineoplastic Agents, Phytogenic / isolation & purification
  • Antineoplastic Agents, Phytogenic / pharmacology*
  • Apoptosis / drug effects*
  • Caulerpa / chemistry*
  • Cell Line, Tumor
  • Cell Proliferation / drug effects
  • Cell Survival / drug effects
  • Chromatin / drug effects
  • Dose-Response Relationship, Drug
  • Female
  • Humans
  • Oligosaccharides / isolation & purification
  • Oligosaccharides / pharmacology*
  • Plant Extracts / chemistry*
  • Poly(ADP-ribose) Polymerase Inhibitors
  • Protein Denaturation
  • Sodium Hydroxide / chemistry
  • Sulfuric Acids / chemistry
  • Xylans / isolation & purification
  • Xylans / pharmacology*

Substances

  • Antineoplastic Agents, Phytogenic
  • Chromatin
  • Oligosaccharides
  • Plant Extracts
  • Poly(ADP-ribose) Polymerase Inhibitors
  • Sulfuric Acids
  • Xylans
  • Sodium Hydroxide
  • 1,3-xylan
  • sulfuric acid