Gene expression profiles induced by E6 from non-European HPV18 variants reveals a differential activation on cellular processes driving to carcinogenesis

Virology. 2012 Oct 10;432(1):81-90. doi: 10.1016/j.virol.2012.05.029. Epub 2012 Jun 27.

Abstract

Cervical cancer in developed countries remains as a major concern on public health policies due to incidence and mortality rates. Persistent infection with high risk human papillomavirus is a necessary etiological agent in the progression to invasive cervical carcinoma. A proposed hypothesis is the association between more aggressive HPV variants and the risk to develop cervical cancer. In order to have a global perspective in terms of cellular transcripts and molecular pathways affected by HPV18 E6 intratype variants; we conducted a genome wide analysis of gene expression. Our results show that E6 derived from non-European variants are able to up-regulate cellular transcripts associated to the hallmarks of cancer; such as cell cycle, migration, Wnt pathway and mTor signaling. Moreover, we were able to show that HPV18 E6 from African variant had a major effect on cellular processes such as cell cycle and migration as confirmed by functional studies.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA-Binding Proteins / metabolism*
  • Female
  • Gene Expression Profiling*
  • Host-Pathogen Interactions*
  • Human papillomavirus 18 / isolation & purification
  • Human papillomavirus 18 / pathogenicity*
  • Humans
  • Oncogene Proteins, Viral / metabolism*
  • Papillomavirus Infections / virology
  • Uterine Cervical Neoplasms / virology
  • Virulence Factors / metabolism*

Substances

  • DNA-Binding Proteins
  • E6 protein, Human papillomavirus type 18
  • Oncogene Proteins, Viral
  • Virulence Factors