Abstract
This study examined the hepatoprotective effect of the HV-P411 complex, an herbal extract mixture from the seeds of Vitis vinifera, Schisandra chinensis and Taraxacum officinale, against D-galactosamine (D-GalN)-induced hepatitis. Hepatotoxicity was induced by D-GalN (700 mg/kg, i.p.), and the HV-P411 complex was administered orally 48, 24, and 2 h before and 6 h after D-GalN injection. Increases in serum aminotransferase activity and lipid peroxidation and a decrease in hepatic glutathione content were attenuated by the HV-P411 complex 24 h after D-GalN treatment. The HV-P411 complex attenuated the increases in serum tumor necrosis factor-α, interleukin (IL)-6 level and cyclooxygenase-2 protein production and their mRNA expressions, while increases in serum IL-10 level and heme oxygenase-1 protein production and their mRNA expressions were augmented by the HV-P411 complex. The increased translocation of nuclear factor-κB and c-Jun phosphorylation were attenuated by treatment with the HV-P411 complex. Our results suggest that the HV-P411 complex prevents D-GalN-induced hepatotoxicity via antioxidative and anti-inflammatory activities.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Anti-Inflammatory Agents / pharmacology
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Anti-Inflammatory Agents / therapeutic use*
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Antioxidants / metabolism
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Antioxidants / pharmacology
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Antioxidants / therapeutic use*
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Chemical and Drug Induced Liver Injury / drug therapy*
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Chemical and Drug Induced Liver Injury / metabolism
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Cyclooxygenase 2 / genetics
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Cyclooxygenase 2 / metabolism
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Drugs, Chinese Herbal / pharmacology
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Drugs, Chinese Herbal / therapeutic use*
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Galactosamine
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Glutathione / metabolism
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Heme Oxygenase-1 / genetics
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Heme Oxygenase-1 / metabolism
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Interleukin-10 / blood
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Interleukin-10 / genetics
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Interleukin-6 / blood
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Interleukin-6 / genetics
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Interleukin-6 / metabolism
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Lipid Peroxidation / drug effects
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Liver / drug effects*
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Liver / metabolism
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Magnoliopsida*
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Male
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NF-kappa B / metabolism
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Phosphorylation
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Phytotherapy*
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Plants, Medicinal
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Proto-Oncogene Proteins c-jun / metabolism
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RNA, Messenger / metabolism
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Rats
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Rats, Sprague-Dawley
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Schisandra
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Seeds
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Taraxacum
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Tumor Necrosis Factor-alpha / metabolism
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Vitis
Substances
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Anti-Inflammatory Agents
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Antioxidants
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Drugs, Chinese Herbal
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Interleukin-6
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NF-kappa B
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Proto-Oncogene Proteins c-jun
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RNA, Messenger
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Tumor Necrosis Factor-alpha
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Interleukin-10
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Galactosamine
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Heme Oxygenase-1
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Cyclooxygenase 2
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Glutathione