Abstract
A variable-number tandem-repeat (VNTR) typing assay for the differentiation of Mycobacterium abscessus strains was developed. This assay showed complete reproducibility, locus stability, and a discriminatory power (Hunter-Gaston discriminatory index [HGDI] of 0.9563) that is superior to that of multilocus sequencing. It is a promising tool for the investigation of Mycobacterium abscessus epidemiology and nosocomial outbreaks.
Publication types
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Comparative Study
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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DNA, Bacterial / genetics
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Humans
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Minisatellite Repeats*
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Molecular Epidemiology / methods
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Molecular Typing / methods*
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Mycobacterium / classification*
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Mycobacterium / genetics*
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Mycobacterium / isolation & purification
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Mycobacterium Infections, Nontuberculous / microbiology
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Polymorphism, Genetic
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Reproducibility of Results
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Sensitivity and Specificity