Abstract
The effect of substrate-mediated signals on osteogenic differentiation of hMSCs is studied using a synthetic bone-like material comprising both organic and inorganic components that supports adhesion, spreading, and proliferation of hMSCs. hMSCs undergo osteogenic differentiation even in the absence of osteogenesis-inducing supplements. They exhibit higher expressions of Runx2, BSP, and OCN compared to their matrix-rigidity-matched, non-mineralized hydrogel counterparts. The mineralized-hydrogel-assisted osteogenic differentiation of hMSCs could be attributed to their exposure to high local concentrations of calcium and phosphate ions in conjunction with chemical and topological cues arising from the hydrogel-bound calcium phosphate mineral layer.
Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Acrylates / chemistry*
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Alkaline Phosphatase / metabolism
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Biomarkers / metabolism
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Calcium Phosphates / chemistry*
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Cell Adhesion / drug effects
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Cell Differentiation / drug effects
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Cell Proliferation / drug effects
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Cells, Cultured
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Cellular Microenvironment
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Coated Materials, Biocompatible / chemistry*
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Coated Materials, Biocompatible / pharmacology
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Core Binding Factor Alpha 1 Subunit / metabolism
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Humans
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Hydrogels
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Integrin-Binding Sialoprotein / metabolism
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Mesenchymal Stem Cells / cytology*
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Mesenchymal Stem Cells / drug effects
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Mesenchymal Stem Cells / physiology
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Osteoblasts / cytology*
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Osteoblasts / drug effects
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Osteoblasts / physiology
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Osteogenesis / drug effects
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Tissue Engineering
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Tissue Scaffolds
Substances
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Acrylates
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Biomarkers
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Calcium Phosphates
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Coated Materials, Biocompatible
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Core Binding Factor Alpha 1 Subunit
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Hydrogels
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Integrin-Binding Sialoprotein
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RUNX2 protein, human
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calcium phosphate
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Alkaline Phosphatase