Abstract
Imaging of the microtubular network is an important strategy to define cell-cycle specific and pathological states of eukaryotic cells. Here, we describe TubStain, a novel recombinant polypeptide allowing direct, non-antibody dependent labeling of microtubules in fixed cells over a broad range of different species and tissues. TubStain has, due to its small size and susceptibility for biochemical and genetic manipulations, high potential as a microtubule marker in state-of-the-art microscopy.
MeSH terms
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Amino Acid Sequence
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Animals
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Cell Division
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Fibroblasts / metabolism
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Humans
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Membrane Proteins / chemistry
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Membrane Proteins / metabolism
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Microtubules
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Molecular Sequence Data
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Peptides / chemistry*
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Peptides / metabolism
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Protein Engineering
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Rats
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Recombinant Fusion Proteins / chemistry*
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Repressor Proteins / chemistry
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Repressor Proteins / metabolism
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Tubulin / chemistry*
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Tubulin / metabolism
Substances
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Membrane Proteins
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PEX14 protein, human
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Peptides
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Recombinant Fusion Proteins
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Repressor Proteins
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Tubulin