Occupational exposure to formaldehyde and alterations in lymphocyte subsets

Am J Ind Med. 2013 Feb;56(2):252-7. doi: 10.1002/ajim.22088. Epub 2012 Jul 5.

Abstract

Background: Formaldehyde is used in many occupational settings, most notably in manufacturing, health care, and embalming. Formaldehyde has been classified as a human carcinogen, but its mechanism of action remains uncertain.

Methods: We carried out a cross-sectional study of 43 formaldehyde-exposed workers and 51 unexposed age and sex-matched controls in Guangdong, China to study formaldehyde's early biologic effects. To follow up our previous report that the total lymphocyte count was decreased in formaldehyde-exposed workers compared with controls, we evaluated each major lymphocyte subset (i.e., CD4(+) T cells, CD8(+) T cells, natural killer [NK] cells, and B cells) and T cell lymphocyte subset (CD4(+) naïve and memory T cells, CD8(+) naïve and memory T cells, and regulatory T cells). Linear regression of each subset was used to test for differences between exposed workers and controls, adjusting for potential confounders.

Results: Total NK cell and T cell counts were about 24% (P = 0.037) and 16% (P = 0.0042) lower, respectively, among exposed workers. Among certain T cell subsets, decreased counts among exposed workers were observed for CD8(+) T cells (P = 0.026), CD8(+) effector memory T cells (P = 0.018), and regulatory T cells (CD4(+) FoxP3(+) : P = 0.04; CD25(+) FoxP3(+) : P = 0.008).

Conclusions: Formaldehyde-exposed workers experienced decreased counts of NK cells, regulatory T cells, and CD8(+) effector memory T cells; however, due to the small sample size; these findings need to be confirmed in larger studies.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Air Pollutants, Occupational / adverse effects*
  • Air Pollutants, Occupational / analysis
  • B-Lymphocytes / metabolism
  • Biomarkers / blood
  • CD8-Positive T-Lymphocytes / metabolism
  • Chemical Industry*
  • Cross-Sectional Studies
  • Female
  • Formaldehyde / adverse effects*
  • Formaldehyde / analysis
  • Humans
  • Killer Cells, Natural / metabolism
  • Linear Models
  • Lymphocyte Count
  • Lymphocyte Subsets / metabolism*
  • Male
  • Matched-Pair Analysis
  • Occupational Exposure / adverse effects*
  • Occupational Exposure / analysis
  • T-Lymphocytes, Regulatory / metabolism

Substances

  • Air Pollutants, Occupational
  • Biomarkers
  • Formaldehyde