Association of P2X7 receptor polymorphisms with bone mineral density and osteoporosis risk in a cohort of Dutch fracture patients

Osteoporos Int. 2013 Apr;24(4):1235-46. doi: 10.1007/s00198-012-2059-x. Epub 2012 Jul 10.

Abstract

The P2X7 receptor is thought to be involved in bone physiology in a pro-osteogenic manner. Therefore, we examined associations between genetic variations in the P2X7 receptor gene and bone mineral density (BMD). We found an association between four non-synonymous polymorphism of the human P2X7 receptor and the risk of osteoporosis.

Introduction: The purpose of this study was to determine whether genetic variation in the P2X7 receptor gene (P2RX7) is associated with decreased BMD and risk of osteoporosis in fracture patients.

Methods: Six hundred ninety women and 231 men aged≥50 years were genotyped for 15 non-synonymous P2RX7 SNPs. BMD was measured at the total hip, lumbar spine and femoral neck.

Results: Four non-synonymous SNPs were associated with BMD. The Ala348Thr gain-of-function polymorphism was associated with increased BMD values at the lumbar spine (p=0.012). Decreased hip BMD values were associated with two loss-of-function SNPs in the P2RX7, i.e., in subjects homozygous for the Glu496Ala polymorphism as well as in subjects carrying at least one variant allele of the Gly150Arg polymorphism (p=0.018 and p=0.011; respectively). In men, we showed that subjects either heterozygous or homozygous for the Gln460Arg gain-of-function polymorphism in the P2RX7 had a significantly 40% decrease in risk of a lower T-score value (OR=0.58 [95%CI, 0.33-1.00]).

Conclusion: Thus, genetic aberrations of P2X7R function are associated with lower BMD and increased osteoporosis risk. Therefore, detection of non-synonymous SNPs within the P2RX7 might be useful for osteoporosis risk estimation at an early stage, potentially enabling better osteoporosis prevention and treatment.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Bone Density / genetics*
  • Female
  • Femur Neck / physiopathology
  • Gene Frequency
  • Genetic Predisposition to Disease
  • Genotype
  • Hip Joint / physiopathology
  • Humans
  • Linkage Disequilibrium
  • Lumbar Vertebrae / physiopathology
  • Male
  • Middle Aged
  • Osteoporosis / genetics*
  • Osteoporosis / physiopathology
  • Osteoporosis, Postmenopausal / genetics
  • Osteoporosis, Postmenopausal / physiopathology
  • Osteoporotic Fractures / genetics*
  • Osteoporotic Fractures / physiopathology
  • Polymorphism, Single Nucleotide*
  • Receptors, Purinergic P2X7 / genetics*

Substances

  • Receptors, Purinergic P2X7