Evaluation of oxidative stress among coronary diabetics patients

Acta Biomed. 2011 Dec;82(3):187-96.

Abstract

Objectives: Determination of the superoxide dismutase (SOD), glutathione peroxidase (GPX) and the total antioxidant status (TAS) and evaluation of inflammation by the use of high sensitivity C reactive protein (hs-CRP) among Tunisian coronary diabetic patients.

Materials and methods: We measured the erythrocyte GPX activity and the plasmatic TAS concentration by colorimetric methods, the apolipoproteins [ApoA1, ApoB], hs-CRP and the fibrinogen by immunonephelometry assays.

Results: TAS and GPX were significantly decreased among patients compared to the controls [TAS: 1,14 +/- 0,28 mmol/l vs 1,55 +/- 0,35 mmol/l, GPX: 59,32 +/- 10,72 U/gHb vs 149,19 +/- 30,95 U/gHb]. For the coronary diabetic patients, the TAS is correlated positively with hs-CRP [r= 0,01, p<10(-3)]. Pearson's correlation shows a significantly positive correlation between GPX and TAS among all patients.

Conclusions: Determination of antioxidative defense markers contributes to understanding the effect of stress oxidative on the development, prevention and therapy of cardiovascular disease. (www.actabiomedica.it).

MeSH terms

  • Aged
  • C-Reactive Protein / analysis
  • Cardiovascular Diseases / metabolism*
  • Colorimetry
  • Diabetic Angiopathies / metabolism*
  • Erythrocytes / metabolism
  • Female
  • Glutathione Peroxidase / metabolism
  • Humans
  • Lipid Peroxidation / physiology
  • Male
  • Middle Aged
  • Oxidative Stress*
  • Superoxide Dismutase / metabolism

Substances

  • C-Reactive Protein
  • Glutathione Peroxidase
  • Superoxide Dismutase