Effect of exogenous erythritol on growth and survival of Brucella

Vet Microbiol. 2012 Dec 7;160(3-4):513-6. doi: 10.1016/j.vetmic.2012.06.014. Epub 2012 Jun 20.

Abstract

Erythritol has been considered as an important factor for the pathogenesis of Brucella abortus 2308 and its ability to cause abortion in ruminants. There is a lack of laboratory models to study the Brucella-erythritol relationship, as commonly used murine models do not have erythritol. We tested the effect of exogenous erythritol on the growth of Brucella in iron minimal medium (IMM), in infected macrophage culture and in infected mice to determine if these models can be used to study the relationship between Brucella and erythritol. An effect of erythritol on Brucella growth was only seen in IMM. There appear to be no effect of erythritol on Brucella growth in macrophage cell cultures or in mice. This shows that administration of erythritol to the mice or macrophages cannot mimic the environment in ruminants during pregnancy and thus cannot be used as models to understand the effect of erythritol on Brucella pathogenesis.

MeSH terms

  • Animals
  • Brucella abortus / drug effects*
  • Brucella abortus / growth & development
  • Brucellosis / microbiology*
  • Culture Media / chemistry
  • Disease Models, Animal
  • Erythritol / pharmacology*
  • Iron / pharmacology
  • Lethal Dose 50
  • Macrophages / microbiology
  • Mice
  • Mice, Inbred BALB C
  • Microbial Viability / drug effects
  • Vasodilator Agents / pharmacology

Substances

  • Culture Media
  • Vasodilator Agents
  • Iron
  • Erythritol