Abstract
A Planctomyces limnophilus mutant generated using the EZ-Tn5 transposome was found to possess an insertion within pckA, encoding phosphoenolpyruvate carboxykinase. Disruption of pckA expression and elimination of enzymatic activity resulted in poor growth in glucose-free medium, demonstrating a gluconeogenic role for pckA in P. limnophilus.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Culture Media / chemistry
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DNA Transposable Elements*
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Gluconeogenesis
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Glucose / metabolism
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Mutagenesis, Insertional / methods*
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Phosphoenolpyruvate Carboxykinase (ATP) / deficiency*
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Phosphoenolpyruvate Carboxykinase (ATP) / metabolism
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Planctomycetales / enzymology*
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Planctomycetales / genetics
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Planctomycetales / growth & development
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Planctomycetales / metabolism
Substances
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Culture Media
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DNA Transposable Elements
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Phosphoenolpyruvate Carboxykinase (ATP)
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Glucose