Electrophysiological features of the mouse tail nerves and their changes in chemotherapy induced peripheral neuropathy (CIPN)

J Neurosci Methods. 2012 Aug 15;209(2):403-9. doi: 10.1016/j.jneumeth.2012.07.005. Epub 2012 Jul 16.

Abstract

Electrophysiology of tail nerves in rodents has been demonstrated a reliable method to investigate models of peripheral neuropathies. Nevertheless, data concerning mouse models are lacking. We assessed the normal features of sensory and motor conduction of tail nerves in adult mice. We found that, as in rats, a sensory compound action potential and motor responses could be recorded with the non invasive and highly reliable technique proposed, especially if bipolar derivations were used. We also investigated the changes related to chemotherapy induced peripheral neuropathy (CIPN) after paclitaxel treatment (times 1 and 2), compared to pre-treatment (time 0) and to controls. It was found that only the sensory compound action potential was involved in CIPN, with decrease in amplitude and conduction velocity, suggesting a significant reduction in number of fast conducting fibres and a correspondent increase in the number of slow conducting ones, although the total amount of active myelinated fibres was deemed to be unchanged through time 0, time 1 and time 2. The results obtained in CIPN provide new functional evidence about the involvement of sensory fibres and may help in better understanding the underlying mechanisms.

MeSH terms

  • Animals
  • Antineoplastic Agents, Phytogenic / adverse effects*
  • Biophysics
  • Disease Models, Animal
  • Electric Stimulation
  • Mice
  • Mice, Inbred C57BL
  • Mice, Inbred DBA
  • Motor Activity / drug effects
  • Neural Conduction / physiology*
  • Paclitaxel / adverse effects*
  • Peripheral Nervous System Diseases / chemically induced*
  • Peripheral Nervous System Diseases / physiopathology*
  • Reaction Time / drug effects
  • Rotarod Performance Test
  • Species Specificity
  • Tail / innervation*
  • Time Factors

Substances

  • Antineoplastic Agents, Phytogenic
  • Paclitaxel