Segregation of donor cell mitochondrial DNA in gaur-bovine interspecies somatic cell nuclear transfer embryos, fetuses and an offspring

Mitochondrion. 2012 Sep;12(5):506-13. doi: 10.1016/j.mito.2012.07.108. Epub 2012 Jul 21.

Abstract

The fate of foreign mitochondrial DNA (mtDNA) following somatic cell nuclear transfer (SCNT) is still controversial. In this study, we examined the transmission of the heteroplasmic mtDNA of gaur donor cells and recipient bovine oocytes to an offspring and aborted and mummified fetuses at various levels during the development of gaur-bovine interspecies SCNT (iSCNT) embryos. High levels of the donor cell mtDNA were found in various tissue samples but they did not have any beneficial effect to the survival of iSCNT offspring. However, the factors on mtDNA inheritance are unique for each iSCNT experiment and depend on the recipient oocyte and donor cell used, which might play an important role in the efficiency of iSCNT.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cattle
  • Cloning, Organism
  • DNA, Mitochondrial*
  • Embryonic Development
  • Female
  • Fetus
  • Gene Transfer, Horizontal*
  • Male
  • Nuclear Transfer Techniques*
  • Ruminants / genetics*

Substances

  • DNA, Mitochondrial