[Effect of change in activity level of catecholaminergic systems on motor, respiratory, and cardiac activities in rat embryos]

Zh Evol Biokhim Fiziol. 2012 May-Jun;48(3):258-67.
[Article in Russian]

Abstract

Parameters of motor, respiratory and cardiac activities were studied in rat embryos (E17-20) after changes in activity level of catecholaminergic systems. To produce conditions for excessive level of catecholamines, the animal were administered individually with preparation of L-DOPA at doses of 25, 50 and 100 mg/kg. Also studied was action of L-DOPA after blockade of D1-(antagonist - SCH-23390, 0.1 mg/kg), D2-(antagonist - sulpiride, 50 mg/kg) dopaminic, and beta2-(antagonist - propranolol, 1 mg/kg) adrenergic receptors. It was found out in E17-18 that the DOPA administration regardless of dose, while in E19-20 dose-dependently produces continuous generalized activity. Between E18 and E19, ontogenetically new is the appearance in 92 % of embryos of stereotypical head movements (circular movements, lateral and dorso-ventral flexions) following in the nearsecond rhythm. Injection of DOPA to rat embryos increased 2-6 times the number of respiratory movements by the gasping type in E17-20 and decreased the amount of episodes of continuous rhythmical respiration in E19-20. No significant heart rate changes were observed after introduction of DOPA to E17-20. There was noted a tendency for a weak acceleration of the heart rate. The changes in activities of the motor and respiratory systems due to a rise of catecholamine level are not connected with activation of the dopamine system, as they are not reduced by blockade of dopamine receptors.

Publication types

  • English Abstract

MeSH terms

  • Adrenergic beta-Antagonists / pharmacology
  • Animals
  • Benzazepines / pharmacology
  • Catecholamines / metabolism*
  • Dopamine D2 Receptor Antagonists
  • Embryo, Mammalian / drug effects*
  • Embryo, Mammalian / metabolism
  • Female
  • Heart Rate / drug effects
  • Levodopa / administration & dosage*
  • Male
  • Motor Activity / drug effects*
  • Pregnancy
  • Rats
  • Receptors, Dopamine D1 / antagonists & inhibitors
  • Receptors, Dopamine D1 / metabolism
  • Receptors, Dopamine D2 / metabolism
  • Respiration / drug effects*
  • Sulpiride / pharmacology

Substances

  • Adrenergic beta-Antagonists
  • Benzazepines
  • Catecholamines
  • Dopamine D2 Receptor Antagonists
  • Receptors, Dopamine D1
  • Receptors, Dopamine D2
  • SCH 23390
  • Levodopa
  • Sulpiride