Abstract
Chikungunya virus nonstructural protein nsP3 has an essential but unknown role in alphavirus replication and interacts with Ras-GAP SH3 domain-binding protein (G3BP). Here we describe the first known function of nsP3, to inhibit stress granule assembly by recruiting G3BP into cytoplasmic foci. A conserved SH3 domain-binding motif in nsP3 is essential for both nsP3-G3BP interactions and viral RNA replication. This study reveals a novel role for nsP3 as a regulator of the cellular stress response.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Alphavirus Infections / metabolism
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Alphavirus Infections / virology*
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Amino Acid Motifs
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Animals
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Chikungunya Fever
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Chikungunya virus / genetics*
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Chlorocebus aethiops
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Conserved Sequence
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Cytoplasm / metabolism*
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Gene Deletion
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Models, Genetic
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Protein Binding
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Protein Structure, Tertiary
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RNA, Viral / metabolism
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Vero Cells
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Viral Nonstructural Proteins / genetics*
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Virus Replication
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src Homology Domains
Substances
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RNA, Viral
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Viral Nonstructural Proteins
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nsp3 protein, alphavirus