Development of 12 microsatellite markers for Platycrater arguta (Hydrangeaceae) endemic to East Asia

Am J Bot. 2012 Aug;99(8):e304-6. doi: 10.3732/ajb.1100582. Epub 2012 Jul 26.

Abstract

Premise of the study: Microsatellite markers were characterized in Platycrater arguta, a rare endemic shrub from eastern China and southern Japan, to investigate its population genetic structure, phylogeographic patterns, and breeding system.

Methods and results: Using both the Fast Isolation by AFLP of Sequences COntaining repeats (FIASCO) protocol and the compound microsatellite marker technique, we developed 12 microsatellite markers. All primers showed polymorphism when assessed in 43 individuals from two populations in eastern China and southern Japan. Overall, the number of alleles ranged from five to 21, with an average of 14.91 alleles per locus.

Conclusions: These markers can be used in further studies on population genetics, phylogeography, and breeding system of P. arguta.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alleles
  • Amplified Fragment Length Polymorphism Analysis
  • Base Sequence
  • Breeding
  • China
  • DNA Primers / genetics
  • DNA, Plant / genetics
  • Genetic Loci
  • Genetic Markers
  • Heterozygote
  • Hydrangeaceae / classification
  • Hydrangeaceae / genetics*
  • Japan
  • Microsatellite Repeats / genetics*
  • Molecular Sequence Data
  • Phylogeography
  • Plant Leaves / classification
  • Plant Leaves / genetics
  • Polymorphism, Genetic*
  • Sequence Analysis, DNA

Substances

  • DNA Primers
  • DNA, Plant
  • Genetic Markers

Associated data

  • GENBANK/JN383518
  • GENBANK/JN383519
  • GENBANK/JN383520
  • GENBANK/JN383521
  • GENBANK/JN383522
  • GENBANK/JN383523
  • GENBANK/JN383524
  • GENBANK/JN383525
  • GENBANK/JN383526
  • GENBANK/JN383527
  • GENBANK/JN383528
  • GENBANK/JN383529