Abstract
YM155, a small-molecule survivin suppressant, specifically binds to the transcription factor ILF3, which regulates the expression of survivin[1]. In this experiment we have demonstrated that p54(nrb) binds to the survivin promoter and regulates survivin expression. p54(nrb) forms a complex with ILF3, which directly binds to YM155. YM155 induces disruption of the ILF3/p54(nrb) complex, which results in a different subcellular localization between ILF3 and p54(nrb). Thus, identification of molecular targets of YM155 in suppression of the survivin pathway, might lead to development of its use as a novel potential target in cancers.
Copyright © 2012 Elsevier Inc. All rights reserved.
MeSH terms
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Active Transport, Cell Nucleus / drug effects
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Cell Line, Tumor
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Cell Nucleolus / metabolism
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DNA-Binding Proteins
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E2F1 Transcription Factor / metabolism
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E2F2 Transcription Factor / metabolism
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HEK293 Cells
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Humans
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Imidazoles / pharmacology*
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Inhibitor of Apoptosis Proteins / biosynthesis*
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Inhibitor of Apoptosis Proteins / genetics
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Naphthoquinones / pharmacology*
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Nuclear Factor 90 Proteins / antagonists & inhibitors*
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Nuclear Factor 90 Proteins / metabolism
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Nuclear Matrix-Associated Proteins / antagonists & inhibitors*
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Nuclear Matrix-Associated Proteins / metabolism
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Octamer Transcription Factors / antagonists & inhibitors*
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Octamer Transcription Factors / metabolism
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Promoter Regions, Genetic
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Protein Binding
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RNA-Binding Proteins / antagonists & inhibitors*
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RNA-Binding Proteins / metabolism
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Survivin
Substances
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BIRC5 protein, human
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DNA-Binding Proteins
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E2F1 Transcription Factor
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E2F1 protein, human
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E2F2 Transcription Factor
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E2F2 protein, human
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ILF3 protein, human
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Imidazoles
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Inhibitor of Apoptosis Proteins
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NONO protein, human
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Naphthoquinones
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Nuclear Factor 90 Proteins
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Nuclear Matrix-Associated Proteins
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Octamer Transcription Factors
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RNA-Binding Proteins
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Survivin
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sepantronium