Discrimination of colon cancer stem cells using noncanonical amino acid

Chem Commun (Camb). 2012 Sep 18;48(72):9035-7. doi: 10.1039/c2cc33776b. Epub 2012 Jul 30.

Abstract

Cancer stem cells (CSCs) may be responsible for tumor recurrence. Metabolic labelling of newly synthesized proteins with non-canonical amino acids allows us to discriminate CSCs in mixed populations due to the quiescent nature of these cells.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alkynes / metabolism*
  • Cell Separation / methods*
  • Colonic Neoplasms / pathology*
  • Glycine / analogs & derivatives*
  • Glycine / metabolism
  • HCT116 Cells
  • Humans
  • Neoplastic Stem Cells / metabolism
  • Neoplastic Stem Cells / pathology*

Substances

  • Alkynes
  • homopropargylglycine
  • Glycine