Detection and molecular characterization of hepatitis E virus in clinical, environmental and putative animal sources

Arch Virol. 2012 Dec;157(12):2363-8. doi: 10.1007/s00705-012-1422-8. Epub 2012 Jul 31.

Abstract

Putative animal reservoirs and environmental samples were studied to investigate potential routes of transmission for indigenous hepatitis E virus (HEV) infection in Hokkaido, Japan. A total of 468 liver samples and 954 environmental samples were collected from 2003 to 2011 for this study. Four swine livers (1 %) were positive for HEV RNA; two strains belonged to genotype 3 and the other two strains were genotype 4. Genotype 3 HEV was detected in a sewage sample and a seawater sample. HEV strains derived from swine liver, seawater and raw sewage samples shared 93-100 % sequence similarity with human HEV strains.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Deer
  • Disease Reservoirs / virology
  • Food Microbiology
  • Genetic Variation*
  • Genotype
  • Hepatitis E / epidemiology
  • Hepatitis E / veterinary*
  • Hepatitis E / virology
  • Hepatitis E virus / classification
  • Hepatitis E virus / genetics*
  • Humans
  • Japan / epidemiology
  • Liver / virology
  • Molecular Sequence Data
  • Norgestrel / analogs & derivatives
  • Ostreidae / virology
  • Phylogeny
  • RNA, Viral / isolation & purification
  • Rivers / virology
  • Seawater / virology
  • Sewage / virology
  • Swine
  • Swine Diseases / epidemiology
  • Swine Diseases / virology*

Substances

  • 3,17-diethynyl-13-ethyl-3,5-gonadiene-17-ol
  • RNA, Viral
  • Sewage
  • Norgestrel

Associated data

  • GENBANK/AB679634
  • GENBANK/AB679635
  • GENBANK/AB679636
  • GENBANK/AB679637
  • GENBANK/AB679638
  • GENBANK/AB682756
  • GENBANK/AB683157
  • GENBANK/AB683158
  • GENBANK/AB683159
  • GENBANK/AB683160
  • GENBANK/AB683161
  • GENBANK/AB683162
  • GENBANK/AB683163
  • GENBANK/AB683164
  • GENBANK/AB683165
  • GENBANK/AB683166
  • GENBANK/AB683167
  • GENBANK/AB683168
  • GENBANK/AB683169
  • GENBANK/AB683170
  • GENBANK/AB683171
  • GENBANK/AB683172
  • GENBANK/AB683173
  • GENBANK/AB683174
  • GENBANK/AB683175
  • GENBANK/AB683176
  • GENBANK/AB683177
  • GENBANK/AB683178
  • GENBANK/AB683179
  • GENBANK/AB683180
  • GENBANK/AB683181
  • GENBANK/AB683182
  • GENBANK/AB683183
  • GENBANK/AB683184
  • GENBANK/AB683185
  • GENBANK/AB683186
  • GENBANK/AB721083
  • GENBANK/AB721084
  • GENBANK/AB721085
  • GENBANK/AB721086