[Influence of angiotensin-(1-7) on cell activation in rat renal interstitial fibroblasts induced by aldosterone]

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2012 Aug;28(8):808-10.
[Article in Chinese]

Abstract

Aim: To explore the influence of angiotensin-(1-7) [Ang-(1-7)] on cell activation and extracellular matrix secretion in rat renal interstitial fibroblasts (NRK-49F) induced by aldosterone (ALD).

Methods: The NRK-49F cells were cultured in vitro, and then were divided into control group, ALD group, Ang-(1-7) group, and ALD+Ang-(1-7) group. When the cells were cultured for 48 h, the expression of α-smooth muscle actin (α-SMA) (a sign of cell activation) was detected by immunocytochemistry; the level of collagen type I (Col I ) in the cultured supernatant was measured by enzyme-linked immunosorbent assay (ELISA). When the cells were cultured for 30 min, the expressions of phosphorylated and total ERK1/2 (pERK1/2, tERK1/2) in the cell lysate were detected by Western blotting.

Results: Compared with control group, the expressions of α-SMA, Col I and the Phos/Total ERK1/2 ratio in ALD group and ALD+Ang-(1-7) group increased significantly (P<0.05). Compared with ALD group, the expressions of α-SMA, Col I and the Phos/Total ERK1/2 ratio in ALD+Ang-(1-7) group decreased significantly (P<0.05).

Conclusion: Ang-(1-7) can inhibit ALD-induced cell activation and decrease the secretion of Col I in rat renal interstitial fibroblasts. Inhibition of ERK1/2 pathway may play an important role in this process.

MeSH terms

  • Actins / metabolism
  • Aldosterone / pharmacology*
  • Angiotensin I / pharmacology*
  • Animals
  • Cell Line
  • Collagen Type I / metabolism
  • Fibroblasts / drug effects*
  • Fibroblasts / metabolism
  • Kidney / cytology*
  • Kidney / drug effects*
  • Kidney / metabolism
  • MAP Kinase Signaling System / drug effects
  • Mitogen-Activated Protein Kinase 1 / metabolism
  • Mitogen-Activated Protein Kinase 3 / metabolism
  • Peptide Fragments / pharmacology*
  • Phosphorylation / drug effects
  • Rats
  • Renin-Angiotensin System

Substances

  • Actins
  • Collagen Type I
  • Peptide Fragments
  • smooth muscle actin, rat
  • Aldosterone
  • Angiotensin I
  • Mitogen-Activated Protein Kinase 1
  • Mitogen-Activated Protein Kinase 3
  • angiotensin I (1-7)