Chemoselective reduction-based fluorescence probe for detection of hydrogen sulfide in living cells

Anal Bioanal Chem. 2012 Oct;404(6-7):1919-23. doi: 10.1007/s00216-012-6292-0. Epub 2012 Aug 7.

Abstract

A selective and sensitive fluorescence probe for hydrogen sulfide (H(2)S) detection was synthesized and evaluated in PBS buffer and fetal bovine serum. The effect of pH on the probe was also studied. In addition, visualization of H(2)S in Hela cells was achieved using confocal laser scanning fluorescence microscopy.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cells / chemistry*
  • Fluorescent Dyes / chemical synthesis
  • Fluorescent Dyes / chemistry*
  • HeLa Cells
  • Humans
  • Hydrogen Sulfide / analysis*
  • Hydrogen-Ion Concentration
  • Oxidation-Reduction
  • Staining and Labeling / instrumentation

Substances

  • Fluorescent Dyes
  • Hydrogen Sulfide