Preparation and evaluation of 99mTc-cefuroxime, a potential infection specific imaging agent: a reliable thin layer chromatographic system to delineate impurities from the 99mTc-antibiotic

Appl Radiat Isot. 2012 Oct;70(10):2384-7. doi: 10.1016/j.apradiso.2012.06.007. Epub 2012 Jun 16.

Abstract

Technetium-99m labelled cefuroxime, a second-generation cephalosporin antibiotic and potential bacteria specific infection imaging agent was evaluated. A good radiochemical purity (95%) of the labelled product was obtained after filtering the reaction mixture through a 0.22 μm filter. Scintigraphy study of the purified product showed uptake in infectious lesions 45 min after injection and abscess-to-muscle ratios were found to be 1.80, 1.85 and 1.88 at 45 min, 1.5 hr and 3 hr, respectively. A versatile and reliable chromatographic technique to assess the radiochemical purity of (99m)Tc-cefuroxime has also been described.

MeSH terms

  • Animals
  • Anti-Bacterial Agents / chemistry*
  • Cefuroxime / chemistry*
  • Organotechnetium Compounds / chemistry*
  • Radionuclide Imaging
  • Rats
  • Rats, Wistar
  • Reproducibility of Results

Substances

  • Anti-Bacterial Agents
  • Organotechnetium Compounds
  • Cefuroxime