Biocompatibility and biodegradability of Mg-Sr alloys: the formation of Sr-substituted hydroxyapatite

Acta Biomater. 2013 Feb;9(2):5319-30. doi: 10.1016/j.actbio.2012.07.045. Epub 2012 Aug 5.

Abstract

Magnesium is an attractive material for use in biodegradable implants due to its low density, non-toxicity and mechanical properties similar to those of human tissue such as bone. Its biocompatibility makes it amenable for use in a wide range of applications from bone to cardiovascular implants. Here we investigated the corrosion rate in simulated body fluid (SBF) of a series of Mg-Sr alloys, with Sr in the range of 0.3-2.5%, and found that the Mg-0.5 Sr alloy showed the slowest corrosion rate. The degradation rate from this alloy indicated that the daily Sr intake from a typical stent would be 0.01-0.02 mg day⁻¹, which is well below the maximum daily Sr intake levels of 4 mg day⁻¹. Indirect cytotoxicity assays using human umbilical vascular endothelial cells indicated that Mg-0.5 Sr extraction medium did not cause any toxicity or detrimental effect on the viability of the cells. Finally, a tubular Mg-0.5 Sr stent sample, along with a WE43 control stent, was implanted into the right and left dog femoral artery. No thrombosis effect was observed in the Mg-0.5 Sr stent after 3 weeks of implantation while the WE43 stent thrombosed. X-ray diffraction demonstrated the formation of hydroxyapatite and Mg(OH)₂ as a result of the degradation of Mg-0.5 Sr alloy after 3 days in SBF. X-ray photoelectron spectroscopy further showed the possibility of the formation of a hydroxyapatite Sr-substituted layer that presents as a thin layer at the interface between the Mg-0.5 Sr alloy and the corrosion products. We believe that this interfacial layer stabilizes the surface of the Mg-0.5 Sr alloy, and slows down its degradation rate over time.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Absorbable Implants*
  • Alloys / pharmacology*
  • Animals
  • Biocompatible Materials / pharmacology*
  • Body Fluids / drug effects
  • Cell Death / drug effects
  • Cell Survival / drug effects
  • Corrosion
  • Dogs
  • Durapatite / pharmacology*
  • Femoral Artery / drug effects
  • Femoral Artery / ultrastructure
  • Human Umbilical Vein Endothelial Cells / cytology
  • Human Umbilical Vein Endothelial Cells / drug effects
  • Humans
  • Hydrogen-Ion Concentration / drug effects
  • Implants, Experimental
  • Materials Testing*
  • Photoelectron Spectroscopy
  • Spectrometry, X-Ray Emission
  • Strontium / pharmacology*
  • Surface Properties
  • X-Ray Diffraction

Substances

  • Alloys
  • Biocompatible Materials
  • Mg-Sr alloy
  • Durapatite
  • Strontium