The role of the alternative coreceptor GPR15 in SIV tropism for human cells

Miriam Kiene; Andrea Marzi; Andreas Urbanczyk; Stephanie Bertram; Tanja Fisch; Inga Nehlmeier; Kerstin Gnirss; Christina B Karsten; David Palesch; Jan Münch; Francesca Chiodi; Stefan Pöhlmann; Imke Steffen

doi:10.1016/j.virol.2012.07.012

The role of the alternative coreceptor GPR15 in SIV tropism for human cells

Virology. 2012 Nov 10;433(1):73-84. doi: 10.1016/j.virol.2012.07.012. Epub 2012 Aug 5.

Authors

Miriam Kiene¹, Andrea Marzi, Andreas Urbanczyk, Stephanie Bertram, Tanja Fisch, Inga Nehlmeier, Kerstin Gnirss, Christina B Karsten, David Palesch, Jan Münch, Francesca Chiodi, Stefan Pöhlmann, Imke Steffen

Affiliation

¹ Institute of Virology, Hannover Medical School, Carl-Neuberg-Str. 1, 30625 Hannover, Germany.

PMID: 22874069
DOI: 10.1016/j.virol.2012.07.012

Abstract

Many SIV isolates can employ the orphan receptor GPR15 as coreceptor for efficient entry into transfected cell lines, but the role of endogenously expressed GPR15 in SIV cell tropism is largely unclear. Here, we show that several human B and T cell lines express GPR15 on the cell surface, including the T/B cell hybrid cell line CEMx174, and that GPR15 expression is essential for SIV infection of CEMx174 cells. In addition, GPR15 expression was detected on subsets of primary human CD4(+), CD8(+) and CD19(+) peripheral blood mononuclear cells (PBMCs), respectively. However, GPR15(+) PBMCs were not efficiently infected by HIV and SIV, including cells from individuals homozygous for the defective Δ32 ccr5 allele. These results suggest that GPR15 is coexpressed with CD4 on PBMCs but that infection of CD4(+), GPR15(+) cells is not responsible for the well documented ability of SIV to infect CCR5(-) blood cells.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antigens, CD / metabolism
B-Lymphocytes / metabolism
B-Lymphocytes / virology*
CCR5 Receptor Antagonists
Cell Line
Humans
RNA, Small Interfering / genetics
Receptors, CCR5 / genetics*
Receptors, CCR5 / metabolism
Receptors, G-Protein-Coupled / antagonists & inhibitors
Receptors, G-Protein-Coupled / genetics*
Receptors, G-Protein-Coupled / metabolism
Receptors, Peptide / antagonists & inhibitors
Receptors, Peptide / genetics*
Receptors, Peptide / metabolism
Receptors, Virus / antagonists & inhibitors
Receptors, Virus / genetics*
Receptors, Virus / metabolism
Simian Immunodeficiency Virus / physiology*
T-Lymphocytes / metabolism
T-Lymphocytes / virology*
Transfection
Viral Tropism / physiology
Virus Internalization
Virus Replication / physiology

Substances

Antigens, CD
CCR5 Receptor Antagonists
GPR15 protein, human
RNA, Small Interfering
Receptors, CCR5
Receptors, G-Protein-Coupled
Receptors, Peptide
Receptors, Virus