[Array-based comparative genomic hybridization detection of copy number variations in a fetus with hypoplastic left-heart syndrome]

Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2012 Aug;29(4):439-42. doi: 10.3760/cma.j.issn.1003-9406.2012.04.013.
[Article in Chinese]

Abstract

Objective: To detect the copy number variations (CNVs) of a fetus with hypoplastic left-heart syndrome, and to assess the value of array-based comparative genomic hybridization (array-CGH) for molecular cytogenetic diagnosis.

Methods: The whole genome of a fetus with normal karyotype by G-banding was scanned and analyzed by array-CGH, and the CNVs was confirmed by multiplex ligation-dependent probe amplification (MLPA).

Results: Two submicroscopic CNVs [del(11)(q24.1-ter)(121951443-134449216, -12.50 Mb),dup(15)(q26.3)(96889082-100215359, -3.33 Mb)] were identified and mapped by array-CGH. MLPA test confirmed both CNVs.

Conclusion: Del (11) (q24.1-ter) may contribute to hypoplastic left-heart syndrome of the fetus. For its high-resolution and high-accuracy, array-CGH has provided a powerful tool for detection of genomic imbalance.

Publication types

  • Case Reports
  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Comparative Genomic Hybridization / methods
  • DNA Copy Number Variations*
  • Female
  • Fetus / metabolism*
  • Humans
  • Hypoplastic Left Heart Syndrome / diagnosis
  • Hypoplastic Left Heart Syndrome / genetics*
  • Hypoplastic Left Heart Syndrome / metabolism
  • Pregnancy
  • Prenatal Diagnosis / methods