Abstract
The effect of sulfated polysaccharides on the efficiency of infection of mouse embryonic fibroblast cell lines SC-1 and NIH-3T3 by replication-competent recombinant Moloney murine leukemia virus (Mo-MuLV) carrying the eGFP gene was investigated. It was shown that used polysaccharides have no cytostatic and cytotoxic effects on SC-1 and NIH 3T3 cells inthe concentrations from 0.01 to 100 μg/ml and have virucidal activity against Mo-MuLV. Polysaccharides in the indicated concentrations inhibit cell infection by Mo-MuLV, that prevents further expansion of viral infection. It was detected that sulfated polysaccharides are effective inhibitors of other retroviruses, including lentiviruses, that use heparan sulfate as cell receptors for non-specific binding.
Publication types
-
English Abstract
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Animals
-
Cell Line
-
Chitosan / analogs & derivatives*
-
Chitosan / chemistry
-
Chitosan / pharmacology*
-
Dose-Response Relationship, Drug
-
Fibroblasts / drug effects
-
Fibroblasts / virology
-
Gene Expression
-
Genes, Reporter
-
Green Fluorescent Proteins / genetics*
-
Heparitin Sulfate / antagonists & inhibitors*
-
Heparitin Sulfate / chemistry
-
Heparitin Sulfate / metabolism
-
High-Throughput Screening Assays
-
Humans
-
Lentivirus / drug effects
-
Lentivirus / physiology
-
Mice
-
Moloney murine leukemia virus / drug effects*
-
Moloney murine leukemia virus / genetics
-
Moloney murine leukemia virus / physiology
-
Receptors, Virus / antagonists & inhibitors*
-
Receptors, Virus / metabolism
-
Transduction, Genetic
-
Virus Replication / drug effects*
Substances
-
Receptors, Virus
-
enhanced green fluorescent protein
-
Green Fluorescent Proteins
-
Chitosan
-
Heparitin Sulfate