Abstract
Here we report a simple procedure for generating colorimetric histone deacetylase (HDAC) substrates by solid-phase peptide synthesis based on racemization-free couplings of amino acid chlorides. We demonstrate the applicability of these substrates in HDAC assays.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Benzenesulfonates / chemical synthesis
-
Benzenesulfonates / chemistry
-
Colorimetry*
-
Histone Deacetylase Inhibitors / chemical synthesis
-
Histone Deacetylase Inhibitors / chemistry
-
Histone Deacetylases / chemistry
-
Histone Deacetylases / metabolism*
-
NAD / chemistry
-
Solid-Phase Synthesis Techniques
-
Substrate Specificity
Substances
-
Benzenesulfonates
-
Histone Deacetylase Inhibitors
-
NAD
-
2,4-dinitrobenzenesulfonic acid
-
Histone Deacetylases