An RNA splicing enhancer that does not act by looping

Helen Lewis; Andrew J Perrett; Glenn A Burley; Ian C Eperon

doi:10.1002/anie.201202932

An RNA splicing enhancer that does not act by looping

Angew Chem Int Ed Engl. 2012 Sep 24;51(39):9800-3. doi: 10.1002/anie.201202932. Epub 2012 Aug 31.

Authors

Helen Lewis¹, Andrew J Perrett, Glenn A Burley, Ian C Eperon

Affiliation

¹ Department of Chemistry, University of Leicester, Leicester, UK.

PMID: 22936639
DOI: 10.1002/anie.201202932

Abstract

Out of the loop: Do the proteins bound to an enhancer site on pre-mRNA interact directly with the splice site by diffusion (looping), as is generally accepted, or does the intervening RNA play a role? By inserting a PEG linker between an enhancer sequence and alternative splice sites, the interaction of these two elements can be studied. Intervening RNA was essential for the enhancer activity, which rules out the looping model.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Base Sequence
Click Chemistry
Enhancer Elements, Genetic*
Humans
Oligonucleotides / chemistry
Oligonucleotides / genetics
Oligonucleotides / metabolism
RNA Precursors / chemistry
RNA Precursors / genetics*
RNA Precursors / metabolism
RNA Splice Sites
RNA Splicing*
RNA, Messenger / chemistry
RNA, Messenger / genetics*
RNA, Messenger / metabolism

Substances

Oligonucleotides
RNA Precursors
RNA Splice Sites
RNA, Messenger