Molecular identification of a rare subtype of Cryptosporidium hominis in infants in China

PLoS One. 2012;7(8):e43682. doi: 10.1371/journal.pone.0043682. Epub 2012 Aug 24.

Abstract

Two Cryptosporidium isolates from separate infants suffering from diarrhea were obtained from a hospital in Zhengzhou, China and were genotyped by PCR amplification and sequence analysis of the small-subunit ribosomal RNA (rRNA) (SSU rRNA), 70-kDa heat shock protein (HSP70), and actin genes. Further subtyping was performed by PCR amplification and sequence analysis of the 60-kDa glycoprotein (gp60) gene. Both the isolates were identified as Cryptosporidium hominis subtype IdA21, a rare subtype previously found only in a human immunodeficiency virus-infected child in South Africa and another child in Jordan.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • China
  • Cryptosporidiosis / genetics*
  • Cryptosporidium / classification
  • Cryptosporidium / genetics*
  • Cryptosporidium / isolation & purification*
  • DNA, Protozoan / genetics
  • Diarrhea / genetics*
  • Humans
  • Infant
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • RNA, Ribosomal, 18S / genetics
  • Sequence Analysis, DNA

Substances

  • DNA, Protozoan
  • RNA, Ribosomal, 18S

Associated data

  • GENBANK/EF570921
  • GENBANK/EF570922
  • GENBANK/EF591783
  • GENBANK/EF591784
  • GENBANK/EF591785
  • GENBANK/EF591786
  • GENBANK/EF591787
  • GENBANK/EF591788

Grants and funding

This study was supported in part by funds for the Key National Science and Technology Specific Projects (no. 2012ZX10004220-001,http://www.nmp.gov.cn/zxjs/crb/201012/t20101208_2127.htm), the National Natural Science Foundation of China (no. 31172311, http://www.nsfc.gov.cn/Portal0/default152.htm), and the Program for Innovative Research Team (in Science and Technology) in University of Henan Province (012IRTSTHN005, http://est.haedu.gov.cn/Article/). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.