Bone marrow-derived HipOP cell population is markedly enriched in osteoprogenitors

Int J Mol Sci. 2012;13(8):10229-10235. doi: 10.3390/ijms130810229. Epub 2012 Aug 16.

Abstract

We recently succeeded in purifying a novel multipotential progenitor or stem cell population from bone marrow stromal cells (BMSCs). This population exhibited a very high frequency of colony forming units-osteoblast (CFU-O; 100 times higher than in BMSCs) and high expression levels of osteoblast differentiation markers. Furthermore, large masses of mineralized tissue were observed in in vivo transplants with this new population, designated highly purified osteoprogenitors (HipOPs). We now report the detailed presence and localization of HipOPs and recipient cells in transplants, and demonstrate that there is a strong relationship between the mineralized tissue volume formed and the transplanted number of HipOPs.

Keywords: bone marrow stromal cell; mesenchymal stem cell; osteoprogenitor; tissue reconstruction; transplantation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bone Marrow Transplantation*
  • Cell Differentiation*
  • Female
  • Mesenchymal Stem Cells / cytology*
  • Mice
  • Mice, Inbred C57BL
  • Mice, SCID
  • Mice, Transgenic
  • Osteoblasts / cytology*
  • Osteogenesis / physiology*
  • Stem Cells / cytology*