To allow processing of serum samples from animals experimentally infected with Coxiella burnetii outside the BSL-3 facility, an inactivation or clearance protocol that does not hamper serological testing may be required. The effects of filtration (0.1 µm pore size), heating at 56 °C for 30 min, addition of NaN3 (0.09% w/v), and combinations thereof on the presence of viable C. burnetii as well as OD-values in ELISA were tested. Only filtration was shown to effectively clear all culturable C. burnetii.
Keywords: Biosafety; Coxiella burnetii; ELISA; Inactivation; Serum.
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