Identification of two paralogous caprine CD36 genes that display highly divergent mRNA expression profiles

Comp Immunol Microbiol Infect Dis. 2013 Jan;36(1):1-7. doi: 10.1016/j.cimid.2012.08.003. Epub 2012 Sep 16.

Abstract

The CD36 molecule plays a pivotal role in a variety of immunological and cellular processes, including pathogen recognition, inflammation and apoptosis. Herein, we demonstrate that this gene is duplicated in goats, with two copies (CD36 and CD36-like) that display highly divergent mRNA expression profiles. In this way, CD36 mRNA is mostly expressed in the adipose tissue and heart whilst CD36-like mRNA shows a high expression in the liver. We have also found evidence of the presence of two paralogous CD36 and CD36-like genes in the bovine genome, suggesting that CD36 duplication took place before goat-cattle radiation, i.e. at least 20 MYR ago. Finally, we have characterized the polymorphism of the coding regions of the goat CD36 and CD36-like genes. In doing so, we have identified one synonymous polymorphism at the CD36-like gene (c.390A>C) that displays a significant association (P=0.04) with milk somatic cell count, a parameter often used to diagnose mastitis in domestic ruminants.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alleles
  • Amino Acid Sequence
  • Animals
  • CD36 Antigens / chemistry
  • CD36 Antigens / genetics*
  • CD36 Antigens / metabolism
  • Female
  • Gene Expression Profiling*
  • Gene Frequency
  • Genetic Loci
  • Genome
  • Genotype
  • Goats / genetics
  • Goats / metabolism
  • Milk / cytology
  • Phylogeny
  • Polymorphism, Genetic
  • RNA, Messenger / metabolism

Substances

  • CD36 Antigens
  • RNA, Messenger