Abstract
In two outbreaks of food-borne botulism in France, Clostridium botulinum type A was isolated and characterized from incriminated foods. Botulinum neurotoxin type A was detected in the patients' sera by mouse bioassay and in vitro endopeptidase assay with an immunocapture step and identification of the cleavage products by mass spectrometry.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Biological Assay / methods
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Botulinum Toxins, Type A / blood*
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Botulism / diagnosis*
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Botulism / epidemiology*
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DNA, Bacterial / chemistry
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DNA, Bacterial / genetics
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Disease Models, Animal
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Disease Outbreaks*
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Foodborne Diseases / diagnosis
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Foodborne Diseases / epidemiology
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France / epidemiology
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Humans
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Mass Spectrometry / methods*
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Mice
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Molecular Sequence Data
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Sequence Analysis, DNA
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Serum / chemistry*
Substances
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DNA, Bacterial
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Botulinum Toxins, Type A
Associated data
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GENBANK/JQ954969
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GENBANK/JQ954970