Rapid low-cost detection of Klebsiella pneumoniae carbapenemase genes by internally controlled real-time PCR

Lijun Wang; Haitong Gu; Xinxin Lu

doi:10.1016/j.mimet.2012.09.009

Rapid low-cost detection of Klebsiella pneumoniae carbapenemase genes by internally controlled real-time PCR

J Microbiol Methods. 2012 Dec;91(3):361-3. doi: 10.1016/j.mimet.2012.09.009. Epub 2012 Sep 17.

Authors

Lijun Wang¹, Haitong Gu, Xinxin Lu

Affiliation

¹ Department of Laboratory Medicine, Beijing Tongren Hospital, Capital Medical University, Beijing, China.

PMID: 22995713
DOI: 10.1016/j.mimet.2012.09.009

Abstract

An internally controlled real-time PCR assay based on SYBR Green was developed to screen Klebsiella pneumoniae carbapenemases (KPCs) gene containing bacteria and was validated for clinical strains or surveillance specimens. When 248 clinical samples were tested, the sensitivity and specificity of the assay were 100% and 99%, respectively.

Publication types

Evaluation Study

MeSH terms

Bacterial Proteins / genetics*
Humans
Klebsiella Infections / microbiology*
Klebsiella pneumoniae / enzymology*
Klebsiella pneumoniae / genetics
Klebsiella pneumoniae / isolation & purification
Real-Time Polymerase Chain Reaction / economics
Real-Time Polymerase Chain Reaction / methods*
Sensitivity and Specificity
beta-Lactamases / genetics*

Substances

Bacterial Proteins
beta-Lactamases
carbapenemase