A potent and selective S1P(1) antagonist with efficacy in experimental autoimmune encephalomyelitis

Chem Biol. 2012 Sep 21;19(9):1142-51. doi: 10.1016/j.chembiol.2012.07.016.

Abstract

Lymphocyte trafficking is critically regulated by the Sphingosine 1-phosphate receptor-1 (S1P(1)), a G protein-coupled receptor that has been highlighted as a promising therapeutic target in autoimmunity. Fingolimod (FTY720, Gilenya) is a S1P(1) receptor agonist that has recently been approved for the treatment of multiple sclerosis (MS). Here, we report the discovery of NIBR-0213, a potent and selective S1P(1) antagonist that induces long-lasting reduction of peripheral blood lymphocyte counts after oral dosing. NIBR-0213 showed comparable therapeutic efficacy to fingolimod in experimental autoimmune encephalomyelitis (EAE), a model of human MS. These data provide convincing evidence that S1P(1) antagonists are effective in EAE. In addition, the profile of NIBR-0213 makes it an attractive candidate to further study the consequences of S1P(1) receptor antagonism and to differentiate the effects from those of S1P(1) agonists.

MeSH terms

  • Administration, Oral
  • Aniline Compounds / administration & dosage
  • Aniline Compounds / chemistry
  • Aniline Compounds / pharmacology*
  • Aniline Compounds / therapeutic use*
  • Animals
  • CHO Cells
  • Cricetinae
  • Cricetulus
  • Dipeptides / administration & dosage
  • Dipeptides / chemistry
  • Dipeptides / pharmacology*
  • Dipeptides / therapeutic use*
  • Disease Models, Animal
  • Dose-Response Relationship, Drug
  • Encephalomyelitis, Autoimmune, Experimental / drug therapy*
  • Encephalomyelitis, Autoimmune, Experimental / pathology
  • Female
  • Humans
  • Leukocytes, Mononuclear / drug effects
  • Lymphocyte Count
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL
  • Molecular Structure
  • Rats
  • Rats, Inbred Lew
  • Rats, Wistar
  • Receptors, Lysosphingolipid / antagonists & inhibitors*
  • Sphingosine-1-Phosphate Receptors
  • Structure-Activity Relationship
  • Substrate Specificity

Substances

  • Aniline Compounds
  • Dipeptides
  • NIBR-0213
  • Receptors, Lysosphingolipid
  • S1PR1 protein, human
  • Sphingosine-1-Phosphate Receptors