Abstract
Morpholino oligomers (MOs) have been widely used to knock down specific genes in zebrafish, but their constitutive activities limit their experimental applications for studying a gene with multiple functions or within a gene network. We report herein a new design and synthesis of caged circular MOs (caged cMOs) with two ends linked by a photocleavable moiety. These caged cMOs were successfully used to photomodulate β-catenin-2 and no tail expression in zebrafish embryos.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Embryo, Nonmammalian / metabolism
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Fetal Proteins
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Gene Expression Regulation* / radiation effects
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Morpholinos / chemical synthesis
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Morpholinos / chemistry*
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Morpholinos / radiation effects
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Oligodeoxyribonucleotides / chemistry
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Photolysis
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T-Box Domain Proteins / genetics
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T-Box Domain Proteins / metabolism
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Zebrafish / embryology
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Zebrafish / genetics*
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Zebrafish / metabolism
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Zebrafish Proteins / genetics
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Zebrafish Proteins / metabolism
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beta Catenin / genetics
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beta Catenin / metabolism
Substances
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Fetal Proteins
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Morpholinos
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Oligodeoxyribonucleotides
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T-Box Domain Proteins
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Zebrafish Proteins
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beta Catenin
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Brachyury protein