The nature of the second-order phase transition that occurs in URu2Si2 at 17.5 K remains puzzling despite intensive research. A key question emerging in the field is whether a hybridization gap between the renormalized bands can be identified as the "hidden" order parameter. We report on the measurement of a hybridization gap in URu2Si2 employing a spectroscopic technique based on quasiparticle scattering. The differential conductance exhibits an asymmetric double-peak structure, a clear signature for a Fano resonance in a Kondo lattice. The hybridization gap opens well above 17.5 K, indicating that it is not the hidden order parameter. Our results put stringent constraints on the origin of the hidden order transition in URu2Si2 and demonstrate that quasiparticle scattering spectroscopy can probe the band renormalizations in a Kondo lattice via detection of a novel type of Fano resonance.