From quantitative protein complex analysis to disease mechanism

Vision Res. 2012 Dec 15:75:108-11. doi: 10.1016/j.visres.2012.08.016. Epub 2012 Sep 23.

Abstract

Interest in the field of cilia biology and cilia-associated diseases - ciliopathies - has strongly increased over the last few years. Proteomic technologies, especially protein complex analysis by affinity purification-based methods, have been used to decipher various basic but also disease-associated mechanisms. This review focusses on some selected recent studies using affinity purification-based protein complex analysis, thereby exemplifying the great possibilities this technology offers.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Animals
  • Cilia* / chemistry
  • Cilia* / genetics
  • Ciliary Motility Disorders / metabolism*
  • Flagella / chemistry
  • Humans
  • Leber Congenital Amaurosis / etiology
  • Mass Spectrometry
  • Membrane Proteins / analysis*
  • Membrane Proteins / physiology
  • Proteins / analysis*
  • Proteins / genetics
  • Proteins / metabolism
  • Proteomics / methods*
  • Signal Transduction / physiology

Substances

  • Membrane Proteins
  • Proteins