Heterologous expression and manipulation of three tetracycline biosynthetic pathways

Angew Chem Int Ed Engl. 2012 Oct 29;51(44):11136-40. doi: 10.1002/anie.201205426. Epub 2012 Sep 28.

Abstract

A very accommodating host: Three tetracycline biosynthetic pathways were overexpressed and manipulated in the heterologous host Streptomyces lividans K4-114. Through the inactivation of various genes and characterization of the resulting biosynthetic intermediates, new tetracycline-modifying enzymes were identified (see scheme).

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Chlortetracycline / analogs & derivatives*
  • Chlortetracycline / biosynthesis
  • Chlortetracycline / chemistry
  • Chlortetracycline / isolation & purification
  • Chromatography, High Pressure Liquid
  • Molecular Structure
  • Oxytetracycline / biosynthesis*
  • Oxytetracycline / chemistry
  • Oxytetracycline / isolation & purification
  • Streptomyces / chemistry
  • Streptomyces / metabolism
  • Tetracyclines / biosynthesis*
  • Tetracyclines / chemistry
  • Tetracyclines / isolation & purification

Substances

  • Tetracyclines
  • dactylocycline A
  • SF 2575
  • Chlortetracycline
  • Oxytetracycline