Abstract
Two novel isolates of Candida glabrata exhibiting reduced sensitivity to amphotericin B (MIC, 8 μg ml(-1)) were found to be ERG2 mutants, wherein Δ(8)-sterol intermediates comprised >90% of the total cellular sterol fraction. Both harbored an alteration at Thr(121) in ERG2; the corresponding residue (Thr(119)) in Saccharomyces cerevisiae is essential for sterol Δ8-Δ7 isomerization. This constitutes the first report of C. glabrata harboring mutations in ERG2 and exhibiting reduced sensitivity to amphotericin B.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Amphotericin B / pharmacology*
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Antifungal Agents / pharmacology*
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Azoles / pharmacology
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Candida glabrata / drug effects*
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Candida glabrata / genetics*
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Candidiasis / microbiology*
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Drug Resistance, Fungal / genetics*
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Fluconazole / pharmacology
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Fungal Proteins / genetics*
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Microbial Sensitivity Tests
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Molecular Sequence Data
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Mutation
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Pyrimidines / pharmacology
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Saccharomyces cerevisiae / drug effects
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Saccharomyces cerevisiae / genetics
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Steroid Isomerases / genetics*
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Sterols / chemistry
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Triazoles / pharmacology
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Voriconazole
Substances
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Antifungal Agents
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Azoles
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Fungal Proteins
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Pyrimidines
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Sterols
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Triazoles
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Amphotericin B
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Fluconazole
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Steroid Isomerases
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Voriconazole