Topical use of rapamycin in herpetic stromal keratitis

Ocul Immunol Inflamm. 2012 Oct;20(5):354-9. doi: 10.3109/09273948.2012.709575.

Abstract

Purpose: To evaluate and compare the efficacy of rapamycin used topically in a mouse model of herpetic stromal keratitis.

Methods: The corneas were infected with herpes simplex virus type-1 strain KOS. Animals were divided into: control (CG), rapamycin (RAPA), cyclosporine (CsA), and dexamethasone (DEXA). The evolution of the disease was assessed clinically and histologically.

Results: On day 10 postinfection (pi), the RAPA group showed only a significantly lower angiogenic development than the CG. On day 14 pi, the treated groups had significantly lower scores for angiogenesis and necrosis than the CG. Also, on day 14 pi, the RAPA and DEXA groups showed significantly lower histopathological scores compared to the CG.

Conclusions: The topical application of 0.05% rapamycin showed greater efficacy than 0.5% cyclosporine and similar efficacy to 0.1% dexamethasone to minimize the immuno-inflammatory process. Also, rapamycin showed early inhibition of the formation of new vessels.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Administration, Ophthalmic
  • Animals
  • Antiviral Agents / therapeutic use*
  • Corneal Stroma / drug effects
  • Corneal Stroma / pathology
  • Corneal Stroma / virology
  • Cyclosporine / therapeutic use
  • Dexamethasone / therapeutic use
  • Herpesvirus 1, Human / drug effects
  • Keratitis, Herpetic / drug therapy*
  • Keratitis, Herpetic / pathology
  • Keratitis, Herpetic / virology
  • Mice
  • Mice, Inbred BALB C
  • Necrosis / drug therapy
  • Necrosis / virology
  • Neovascularization, Pathologic / drug therapy
  • Neovascularization, Pathologic / pathology
  • Neovascularization, Pathologic / virology
  • Severity of Illness Index
  • Sirolimus / administration & dosage
  • Sirolimus / therapeutic use*
  • Treatment Outcome

Substances

  • Antiviral Agents
  • Dexamethasone
  • Cyclosporine
  • Sirolimus