Complement-dependent pathogenicity of brain-specific antibodies in cerebrospinal fluid

J Neuroimmunol. 2013 Jan 15;254(1-2):76-82. doi: 10.1016/j.jneuroim.2012.09.010. Epub 2012 Sep 29.

Abstract

The specificity and potential pathogenicity of autoantibodies vary between neurological diseases. It is often unclear whether their detection in cerebrospinal fluid (CSF) is a consequence or a cause of pathology. The goal was to test whether administration of brain-specific antibodies into CSF would be sufficient for pathology. Purified immunoglobulin G from a neuromyelitis optica patient was injected intrathecally with complement to naïve mice. Histopathological analysis at 7 days revealed damage to the ependyma, disruption of the CSF parenchymal barrier and pathologic lesions, distant from the site of injection. In the absence of complement there was no pathology. Autoantibody and complement in CSF are thus sufficient to initiate a pathologic cascade.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Aquaporin 4 / immunology*
  • Brain / immunology
  • Brain / metabolism
  • Brain / pathology*
  • Complement System Proteins / cerebrospinal fluid*
  • Complement System Proteins / immunology
  • Complement System Proteins / toxicity
  • Demyelinating Diseases / chemically induced
  • Demyelinating Diseases / immunology
  • Demyelinating Diseases / metabolism
  • Ependyma / pathology
  • Female
  • Glial Fibrillary Acidic Protein / metabolism
  • HEK293 Cells
  • Humans
  • Immunoglobulin G / cerebrospinal fluid*
  • Immunoglobulin G / toxicity
  • Injections, Spinal
  • Mice
  • Mice, Inbred C57BL
  • Neuromyelitis Optica / blood
  • Neuromyelitis Optica / immunology
  • Transfection

Substances

  • AQP4 protein, human
  • Aquaporin 4
  • Glial Fibrillary Acidic Protein
  • Immunoglobulin G
  • Complement System Proteins