A genome wide RNA interference screening method to identify host factors that modulate influenza A virus replication

Methods. 2013 Feb;59(2):217-24. doi: 10.1016/j.ymeth.2012.09.009. Epub 2012 Oct 2.

Abstract

The use of genome wide RNA interference (RNAi) screens to investigate host-virals interactions has revealed unexpected connections that have improved our understanding of viral pathogenesis and cell biology. This work describes the use of an RNAi screening method employing an immunofluorescence image-based strategy and influenza A virus. We find this approach to be readily implemented, scalable and amenable to the direct evaluation of a variety of viral lifecycles.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Genome-Wide Association Study / methods*
  • HeLa Cells
  • High-Throughput Screening Assays / methods*
  • Host-Derived Cellular Factors / genetics
  • Humans
  • Influenza A virus
  • RNA Interference*
  • RNA, Small Interfering / genetics
  • Tissue Culture Techniques
  • Virus Replication / genetics*

Substances

  • Host-Derived Cellular Factors
  • RNA, Small Interfering