Abstract
We evaluated the utility of 5 commercial enzyme-linked immunosorbent assay (ELISA) kits for detecting antibodies to avian influenza viruses. The sensitivities and specificities of the ELISA kits were compared with those of the agar gel precipitation (AGP) and hemagglutination-inhibition (HI) tests. The results suggest that some ELISA kits might not be suitable for monitoring during the early stages of avian influenza virus infections. Therefore, ELISA kits should only be used in conjunction with a profound knowledge about monitoring of avian influenza.
Publication types
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Comparative Study
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Evaluation Study
MeSH terms
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Agar
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Animals
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Antibodies, Viral / isolation & purification*
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Birds
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Enzyme-Linked Immunosorbent Assay / instrumentation
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Enzyme-Linked Immunosorbent Assay / methods
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Enzyme-Linked Immunosorbent Assay / veterinary*
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Gels
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Hemagglutination Inhibition Tests / instrumentation
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Hemagglutination Inhibition Tests / methods
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Hemagglutination Inhibition Tests / veterinary*
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Influenza A Virus, H5N1 Subtype / immunology*
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Influenza in Birds / diagnosis*
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Influenza in Birds / immunology
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Influenza in Birds / virology
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Reagent Kits, Diagnostic / veterinary*
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Reagent Kits, Diagnostic / virology
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Sensitivity and Specificity
Substances
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Antibodies, Viral
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Gels
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Reagent Kits, Diagnostic
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Agar