Long- and short-term treatment with imatinib attenuates the development of chronic kidney disease in experimental anti-glomerular basement membrane nephritis

Nephrol Dial Transplant. 2013 Mar;28(3):576-84. doi: 10.1093/ndt/gfs414. Epub 2012 Oct 8.

Abstract

Background: Imatinib is a selective tyrosine kinase inhibitor that can block platelet-derived growth factor (PDGF) receptor activity. Imatinib is also known as an anti-inflammatory agent. We examined the therapeutic effects of long- or short-term imatinib treatment in Wistar-Kyoto (WKY) rats with established anti-glomerular basement membrane (GBM) nephritis.

Methods: Nephrotoxic serum (NTS) nephritis was induced in WKY rats on day 0. Groups of animals were given either imatinib or vehicle daily by intraperitoneal injection, from day 7 to day 49 in the long-term treatment study, and from day 7 to 13 in the short-term treatment study; all rats were sacrificed at day 50.

Results: In long-term treatment, imatinib showed marked renoprotective effects; imatinib suppressed proteinuria, improved renal function, attenuated the development of glomerulosclerosis and tubulointerstitial injury and reduced the expression levels of collagen type I and transforming growth factor-beta (TGF-β) in renal cortex. The key finding of the present study was that short-term treatment with imatinib also significantly attenuated the development of renal injury until day 50, although the degree of renoprotection was slightly inferior to that of long-term treatment.

Conclusions: These results suggest that administration of imatinib is a promising strategy for limiting the progression of glomerulonephritis (GN) to end-stage renal failure. In particular, a short period of treatment at an early stage of GN is more beneficial in terms of cost-effectiveness and reduction of adverse effects in comparison to a continuous and long period of treatment.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anti-Glomerular Basement Membrane Disease / complications*
  • Anti-Glomerular Basement Membrane Disease / pathology
  • Benzamides / therapeutic use*
  • Collagen Type I / genetics
  • Collagen Type I / metabolism
  • Creatinine / metabolism
  • Disease Progression
  • Female
  • Fluorescent Antibody Technique
  • Imatinib Mesylate
  • Piperazines / therapeutic use*
  • Protein Kinase Inhibitors / therapeutic use*
  • Proteinuria
  • Pyrimidines / therapeutic use*
  • RNA, Messenger / genetics
  • Rats
  • Rats, Inbred WKY
  • Real-Time Polymerase Chain Reaction
  • Renal Insufficiency, Chronic / etiology
  • Renal Insufficiency, Chronic / metabolism
  • Renal Insufficiency, Chronic / pathology
  • Renal Insufficiency, Chronic / prevention & control*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Time Factors
  • Transforming Growth Factor beta / genetics
  • Transforming Growth Factor beta / metabolism

Substances

  • Benzamides
  • Collagen Type I
  • Piperazines
  • Protein Kinase Inhibitors
  • Pyrimidines
  • RNA, Messenger
  • Transforming Growth Factor beta
  • Imatinib Mesylate
  • Creatinine