Fluorescent polymer-based post-translational differentiation and subtyping of breast cancer cells

Analyst. 2012 Dec 7;137(23):5487-90. doi: 10.1039/c2an35877h. Epub 2012 Oct 12.

Abstract

Herein, we report the application of synthesized fluorescent, water soluble polymers for post-translational subtyping and differentiation of breast cancer cells in vitro. The fluorescence emission spectra from these polymers were modulated differently in the presence of conditioned cell culture media from various breast cancer cells. These polymers differentiate at a post-translation level possibly due to their ability to interact with extracellular enzymes that are over-expressed in cancerous conditions.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Breast Neoplasms / classification*
  • Breast Neoplasms / genetics
  • Breast Neoplasms / pathology
  • Cell Line, Tumor
  • Culture Media, Conditioned
  • Drug Resistance, Multiple
  • Drug Resistance, Neoplasm
  • Female
  • Fluorescent Dyes
  • HEK293 Cells
  • HeLa Cells
  • Humans
  • Metalloproteins / analysis
  • Metalloproteins / antagonists & inhibitors
  • Polymers
  • Spectrometry, Fluorescence

Substances

  • Culture Media, Conditioned
  • Fluorescent Dyes
  • Metalloproteins
  • Polymers