Abstract
Oligonucleotides that incorporate a reactive moiety to form an interstrand cross-link have been widely studied for their potential toward inhibiting gene expression or as basic tools for chemical biology studies. The 6-vinylpurine (2) newly designed in the current study serves well as a new purine-base moiety for increasing cross-link reactivity to target cytosine. Thus, oligonucleotides containing 6-vinylpurine exhibit a more selective and much smoother DNA cross-linking ability to cytosine than the oligonucleotide analogs derived from 2-amino-6-vinylpurine (1) previously explored.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Alkaline Phosphatase / chemistry
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Chromatography, High Pressure Liquid
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Cross-Linking Reagents / chemistry*
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Cytosine / chemistry
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DNA / chemical synthesis
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DNA / chemistry*
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DNA / isolation & purification
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DNA Cleavage
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Oligodeoxyribonucleotides / chemical synthesis
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Oligodeoxyribonucleotides / chemistry
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Oligodeoxyribonucleotides / isolation & purification
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Phosphoric Diester Hydrolases / chemistry
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Purines / chemistry*
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Vinyl Compounds / chemistry*
Substances
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2-amino-6-vinylpurine
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Cross-Linking Reagents
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Oligodeoxyribonucleotides
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Purines
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Vinyl Compounds
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Cytosine
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DNA
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Alkaline Phosphatase
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Phosphoric Diester Hydrolases