Hyaluronic acid modified mesoporous silica nanoparticles for targeted drug delivery to CD44-overexpressing cancer cells

Nanoscale. 2013 Jan 7;5(1):178-83. doi: 10.1039/c2nr32145a. Epub 2012 Oct 18.

Abstract

In this paper, a targeted drug delivery system has been developed based on hyaluronic acid (HA) modified mesoporous silica nanoparticles (MSNs). HA-MSNs possess a specific affinity to CD44 over-expressed on the surface of a specific cancer cell line, HCT-116 (human colon cancer cells). The cellular uptake performance of fluorescently labelled MSNs with and without HA modification has been evaluated by confocal microscopy and fluorescence-activated cell sorter (FACS) analysis. Compared to bare MSNs, HA-MSNs exhibit a higher cellular uptake via HA receptor mediated endocytosis. An anticancer drug, doxorubicin hydrochloride (Dox), has been loaded into MSNs and HA-MSNs as drug delivery vehicles. Dox loaded HA-MSNs show greater cytotoxicity to HCT-116 cells than free Dox and Dox-MSNs due to the enhanced cell internalization behavior of HA-MSNs. It is expected that HA-MSNs have a great potential in targeted delivery of anticancer drugs to CD44 over-expressing tumors.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Absorption
  • Antibiotics, Antineoplastic / administration & dosage
  • Antibiotics, Antineoplastic / chemistry
  • Cell Line, Tumor
  • Colonic Neoplasms / drug therapy*
  • Colonic Neoplasms / pathology
  • Diffusion
  • Doxorubicin / administration & dosage*
  • Doxorubicin / chemistry
  • Humans
  • Hyaluronan Receptors / metabolism*
  • Hyaluronic Acid / chemistry*
  • Materials Testing
  • Nanocapsules / administration & dosage*
  • Nanocapsules / chemistry*
  • Nanocapsules / ultrastructure
  • Porosity
  • Silicon Dioxide / chemistry*
  • Treatment Outcome
  • Up-Regulation

Substances

  • Antibiotics, Antineoplastic
  • Hyaluronan Receptors
  • Nanocapsules
  • Silicon Dioxide
  • Doxorubicin
  • Hyaluronic Acid