Abstract
Dynamics of DNA repair and recruitment of repair factors to damaged DNA can be studied by live cell microscopy. DNA damage is usually inflicted by a laser beam illuminating a DNA-interacting photosensitizer in a small area of the nucleus. We demonstrate that a focused beam of visible low intensity light alone can inflict local DNA damage and permit studies of DNA repair, thus avoiding potential artifacts caused by exogenous photosensitizers.
Copyright © 2012 Elsevier B.V. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Cell Nucleus / genetics
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Cell Nucleus / metabolism
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Cell Nucleus / radiation effects
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Chromatin / genetics
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Chromatin / metabolism
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Chromatin / radiation effects*
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Color
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DNA Damage*
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DNA Repair
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / metabolism
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Ethidium / adverse effects
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Fluorescent Antibody Technique
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Genome, Human / radiation effects
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HeLa Cells
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Histones / genetics
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Histones / metabolism
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Humans
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Lasers / adverse effects
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Light*
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Oxidative Stress
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Phosphorylation
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Photosensitizing Agents / adverse effects
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Time Factors
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Transcription Factor TFIIH / genetics
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Transcription Factor TFIIH / metabolism
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X-ray Repair Cross Complementing Protein 1
Substances
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Chromatin
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DNA-Binding Proteins
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H2AX protein, human
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Histones
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Photosensitizing Agents
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X-ray Repair Cross Complementing Protein 1
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Transcription Factor TFIIH
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Ethidium