Transcription-dependent nuclear localization of DAZAP1 requires an N-terminal signal

Biochem Biophys Res Commun. 2012 Nov 23;428(3):422-6. doi: 10.1016/j.bbrc.2012.10.076. Epub 2012 Oct 27.

Abstract

Deleted in Azoospermia Associated Protein 1 (DAZAP1) is a ubiquitous hnRNP protein required for normal development and spermatogenesis. It resides predominantly in the nucleus and moves between the nucleus and the cytoplasm via a ZNS shuttling signal at its C-terminus. DAZAP1 accumulates in the cytoplasm when RNA polymerase II activity is inhibited by actinomycin D. Here we report the mapping of a 42-amino acid segment (N42) at the N-terminus of DAZAP1 that is both necessary and sufficient for its transcription-dependent nuclear localization. In addition, using a yeast two-hybrid system, we have identified SLIRP as a N42-binding protein which may regulate DAZAP1 subcellular localization.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Active Transport, Cell Nucleus
  • Amino Acid Sequence
  • Cell Nucleus / metabolism*
  • Humans
  • Molecular Sequence Data
  • Nuclear Localization Signals / genetics
  • Nuclear Localization Signals / metabolism*
  • Protein Interaction Maps
  • RNA-Binding Proteins / genetics
  • RNA-Binding Proteins / metabolism*
  • Transcription, Genetic*

Substances

  • DAZAP1 protein, human
  • Nuclear Localization Signals
  • RNA-Binding Proteins
  • SLIRP protein, human